Cardiogen – the Heart bioregulator

What is Cardiogen?

Cardiogen (H-Ala-Glu-Asp-Arg-OH) is a synthetic tetrapeptide with a molecular weight of ~489.5 Da, classified as a short bioregulatory peptide. Listed in PubChem (CID: 11583989), it is studied for its regulatory effects on cardiomyocytes, fibroblasts, and cellular gene expression, with applications across cardiovascular, oncology, and age-related cellular research.

Research Overview

Structure and Molecular Characteristics

Cardiogen is a synthetic peptide composed of four amino acids: alanine, glutamic acid, aspartic acid, and arginine. Its molecular formula is C₁₈H₃₁N₇O₉, with a molecular weight of 489.49 g/mol [1]. Classified as a polar tetrapeptide, it exhibits high solubility in aqueous environments, enabling its use in diverse experimental models. Unlike individual amino acids, the peptide demonstrates enhanced stability and cell-penetrating potential, supporting research into tissue-specific regulation [1].

Cardiovascular Research

Studies in organotypic rat heart cultures indicate that Cardiogen supports cardiomyocyte proliferation while reducing fibroblast-mediated extracellular matrix deposition [2]. Experimental models also show that it downregulates p53 activity, suggesting a role in modulating apoptosis signaling in myocardial tissues [3]. Importantly, research highlights that it maintains activity in both young and aged cardiac tissues, unlike individual amino acids, which show diminished effects in senescent models [2]. These findings position it as a candidate for ongoing investigations into cardiac regeneration and age-related myocardial function.

Oncology Research

In senescent rat models bearing M-1 sarcoma transplants, Cardiogen induced apoptotic signaling within tumor cells, disrupted vascular integrity, and promoted hemorrhagic necrosis in a dose-dependent manner [4]. These effects were not attributed to direct cytotoxicity, but rather to vascular network modulation, making it a subject of research into tumor microenvironment regulation and growth inhibition [4].

Prostate and Fibroblast Research

Research into prostate fibroblasts shows that Cardiogen enhances the expression of regulatory molecules diminished by aging, including CXCL12, WFDC1, and ghrelin [5]. These proteins influence fibroblast differentiation and extracellular signaling, which are critical for tissue integrity. Findings suggest that it may restore or support signaling pathways affected by senescence, making it relevant for studies in aging biology and fibroblast regulation [5].

Molecular and Gene Expression Studies

Cardiogen has been shown to modulate structural proteins such as actin, vimentin, tubulin, and lamins A/C [6]. These proteins are vital for cytoskeletal organization and nuclear integrity. Additional research has identified changes in gene expression linked to mitochondrial metabolism, myocardial contractility, and oxidative stress responses [6]. Such regulatory effects make it valuable for systems biology approaches in peptide-mediated gene regulation.

Reconstitution and dosing

Cardiogen is typically reconstituted with Bacteriostatic water in various concentrations, usually at 10mg/ml. It is typically dosed at 2mg per day for 20 days, and this protocol is followed 2-3 times per year for heart and cardiovascular health and support.

For our free Peptide Calculator, CLICK HERE. Research-grade Cardiogen is available from WellnessPeptides.

Referenced Citations

1. PubChem. H-Ala-Glu-Asp-Arg-OH (Cardiogen), Compound Summary, CID 11583989. https://pubchem.ncbi.nlm.nih.gov/compound/11583989
2. “The effect of amino acids and cardiogen on the development of myocardial tissue culture from young and old rats.” Adv Gerontol. https://pubmed.ncbi.nlm.nih.gov/20210190/
3. “The tissue-specific effect of synthetic peptide bioregulators in organotypic tissue culture in young and old rats.” Adv Gerontol. https://pubmed.ncbi.nlm.nih.gov/17152728/
4. “Tumor-modifying effect of cardiogen peptide on M-1 sarcoma in senescent rats.” Bull Exp Biol Med. 2010. https://pubmed.ncbi.nlm.nih.gov/20396706/
5. “Peptidergic regulation of the expression of signal factors of fibroblast differentiation in the human prostate gland in cell aging.” Adv Gerontol. https://pubmed.ncbi.nlm.nih.gov/20586252/
6. Khavinson VKh, Popovich IG, Linkova NS, et al. “Peptide Regulation of Gene Expression: A Systematic Review.” Molecules. 2021;26(22):7053. https://pubmed.ncbi.nlm.nih.gov/34834147/

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